Search results for "Beta-2 adrenergic receptor"

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Biophysical and functional characterization of the human olfactory receptor OR1A1 expressed in a mammalian inducible cell line

2014

International audience; Olfactory receptors (ORs) play a crucial role in detecting the odorant molecules present in the surrounding environment. These receptors, which belong to class A G-protein-coupled receptors, constitute the largest transmembrane protein family in the human genome. Functional studies showed that the OR family includes members that are able to respond to a large set of odorants and members that are activated by a relatively small number of related odorants. To understand the molecular mechanisms that govern the receptor-ligand interactions, we overexpressed the human OR hOR1A1 in a stable tetracycline-inducible HEK293S cell line. This receptor was engineered by insertin…

0301 basic medicineCircular dichroismbindingpurification[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionRecombinant Fusion ProteinsGene Expressionodorant receptorsBiologyReceptors OdorantEpitope03 medical and health sciencesRecombinant expression[SDV.IDA]Life Sciences [q-bio]/Food engineeringmedicineOlfactory receptorHumans[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringReceptorSite-directed mutagenesisagonistLigand bindingComputingMilieux_MISCELLANEOUSbeta(2)-adrenergic receptorOlfactory receptortechnology industry and agricultureStructure[SDV.IDA] Life Sciences [q-bio]/Food engineeringTransmembrane proteinprotein-coupled receptors[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition030104 developmental biologymedicine.anatomical_structureHEK293 CellsBiochemistryCell culturehigh-level expressionmembrane-proteinsBeta-2 adrenergic receptoractivationsite-directed mutagenesis[SDV.AEN]Life Sciences [q-bio]/Food and NutritionBiotechnology

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